# Dilution Calculation Explained

## Understanding Dilution Factor Calculation

**Dilution factor (D.F.)** is an important concept in laboratory settings, particularly in sample preparation and analysis. It represents the ratio of the volume of the original sample to the final volume after dilution.

## Calculating Dilution Factor

When dealing with dilutions, the formula for Dilution factor is straightforward: **D.F = 1/dilution ratio.** For example, if the dilution of a sample is 1/8, the dilution factor would be 1/(1/8) = 8.

## Practical Examples

Let's consider a scenario where you want to make a 1/18 dilution of a sample. If you use 7 ml of the sample, the calculation would be as follows: D.F = 1/(1/18) = 18. The final solution volume can be determined by multiplying the volume from the stock solution by the dilution factor, resulting in 7 ml x 18 = 126 ml. Therefore, you would need 119 ml of diluent to make the 1/18 dilution of the 7 ml stock solution.

Furthermore, in a three-step serial dilution with dilutions of 1/6, 1/2, and 1/10, the final dilution factor can be obtained by multiplying all individual dilution factors together. In this case, the final dilution factor would be 1/6 x 1/2 x 1/10 = 1/120 or D.F = 120.